Influence of Sperm Pretreatment on the Efficiency of Intracytoplasmic Sperm Injection in Pigs

Influence of sperm pretreatment on the efficiency of intracytoplasmic sperm injection in pigs.

The purpose of this study was to determine the influence of sperm pretreatment on the efficiency of intracytoplasmic sperm injection (ICSI) in pigs. This was done by examining the effect of 1) the conservation method (fresh vs frozen); 2) the sperm treatment preinjection (resuspension in Dulbecco phosphate-buffered saline (DPBS) vs selection by a Percoll gradient); and 3) the acrosomal and live...

Intracytoplasmic sperm injection (ICSI).

Intracytoplasmic Sperm Injection

Intracytoplasmic Sperm Injection (ICSI) is an assisted reproductive technique [4] (ART) initially developed by Dr. Gianpiero D. Palermo [5] in 1993 to treat male infertility [6]. It is most commonly used in conjunction with in vitro [7] fertilization [8] (IVF) or a less commonly used technique called zygote intrafallopian transfer [9] (ZIFT). In natural fertilization [8], the sperm [10] must pe...

Intracytoplasmic sperm injection (ICSI)

IVF has become a well-established treatment for certain types of infertility, including long-standing infertility due to tubal disease, endometriosis, unexplained infertility or infertility involving a male factor. Soon it became clear that certain couples with severe male-factor infertility could not be helped by conventional IVF. Extremely low sperm counts, impaired motility and/or abnormal m...

Indications for intracytoplasmic sperm injection.

Intracytoplasmic sperm injection (ICSI) is the latest of several microfertilization techniques that have been utilized predominantly to overcome severe male subfertility, giving fertilization and term pregnancy rates similar to conventional in-vitro fertilization (IVF) (but for other indications). Even though available data on children born after ICSI are very encouraging, the procedure must st...